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KMID : 1101320050370010027
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Korean Journal of Clinical Laboratory Science
2005 Volume.37 No. 1 p.27 ~ p.34
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Bacillus cereus Clinical Isolates : Characteristics, Enterotoxin Production and Antimicrobial Susceptibility
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Kim Shin-Moo
Kim Eun-Cheol
So Hyang-Ah
Lee Gyu-Sik
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Abstract
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Biochemical characteristics, enterotoxin production and antimicrobial susceptibility were determined for 30 strains of Bacillus cereus isolated from stool specimens of diarrhea patients at an university hospital in Chulabuk-do province. Positive rate for VP reaction and citrate utilization were lower, (33 % and 40 % respectively) while the rates of acid production from mannitol, arabinose, and xylose were higher (17 %, 13 % and 3 % respectively) than those obtained by other investigators. The enterotoxin gene was detected in 18 of 30 isolates (60 %) by PCR, and the toxin was detected from all of the toxin gene-positive isolates by RPLA test. The agar dilution test showed that all isolates were resistant to penicillin G and 73 % were to cephalothin, but all were susceptible to ciprofloxacin, clindamycin, erythromycin, fusidic acid, gentamicin, rifampin, teracycline and vancomycin. We conclude that B. cereus isolates producing acid from mannitol, arabinose and xylose exist, that PCR can be used to detect enterotoxin genes rapidly and accurately, and that this organism is susceptible to various antimicrobial agents though not penicillin G and cephalothin.
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KEYWORD
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Bacillus cereus, Enterotoxin, Antimicrobial susceptibility
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